C. elegans Synchronizer
Healthy, Synchronized, Phenotype free worms
The C. elegans Synchronizer is a manual worm Synchronizer that allows you to achieve near absolute synchronization in your L1 harvest with no bleach or chemicals, and with little or no training required. You get healthy worms while increasing the reproducibility of your data.
With the C. elegans Synchronizer, you can:
|- model 5000 -||- model 700 -|
Below results for the CES (v2) protocol versus sedimentation and bleaching protocols are from Nagi Bioscience located at the Swiss Federal Institute of Technology (EPFL). Nagi Bioscience is working on the first Organism-on-Chip technology using C. elegans, creating a technological platform that is fully automated in vitro handling, culture and analysis. If you are interested in the development by Nagi Bioscience of the the first Organism-on-Chip technology using C. elegans, you can reach them by sending an email at
|Figure A. Results showing the reproducibility of the CES to generate synchronous L1 population across 34 replicates. L1 worms were injected and cultivated on chips during 5 days. Each channel (represented on the x-axis) corresponds to 3 to 8 microfluidic chambers containing 1 to 4 worms. Each dots represented on the graphic correspond to the timing (in hours) when the first egg is observed in average in the corresponding channel. The error bars represent the standard deviation.|
|Figure B. Comparison of the percentage of fertile worms between three methods of L1 synchronization. Each bar represented on the graphic correspond to the percentage of chambers (on average) with fertile adult worms. n corresponds to the number of chambers analyzed. The error bars represent the 95% CI.|
|Figure C. Variation of the timing to reach the adult stage across single individuals. Each dot represented on the graphic correspond to the variation of the timing for single worms to reach the adult stage, compared to the whole population analyzed. n corresponds to the number of single worms analyzed. sd corresponds to the value of the standard deviation. All 'CES' worms reach adulthood virtually at the same time, demonstrating the superior level of synchronization of the CES versus the Sedimentation and Bleaching protocol.|
+ How does it work?
Let’s start with what it is NOT. The CES is NOT a sieve trying to separate L1’s from L2, L3 etc. The CES is a system that with a two-step protocol will harvest L1’s at the moment they hatch from the egg’s.
2 step protocol to harvest freshly hatched L1's :
Step 1 Wash: Washing out all the debris and anything smaller than Adults, L1's, L2 up to L4 are removed, only adults and most eggs are kept.
Step 2 Harvest: Adults and eggs are transferred from the Stabilizing filter to the Harvest filter. As soon as the eggs hatch, the L1's will transfer through the filter with little or no delay.
More details can be found at: Newsletter How does it work.
- check the Protocol Demo in below Video section -
+ Does the system works with small amount of worms as well?
Yes, you can use small volumes of a worm culture. Recommended is a minimum of 10 gravid adults including laid eggs. For up to ~1k of L1's to be harvested, the CES-700 is typically recommended. For (very) large volumes of L1's the CES-5000 is most likely more suitable. Please contact us for advice.
+ Can I synchronize multiple strains?
Yes, each strain is synchronized in approximately 10 to 15 minutes. Please keep in mind that each strain will have to remain in the 'Harvest Filter' for a period of time (i.e. 'Harvest Time'). In order to synchronize multiple strains and not having to wait for the Harvest Filter to become available again, it is advised to purchase for each additional strain an extra 'Harvest Filter'. This will allow extended harvest time for each strain. In a typical setup, where one person is working with the Synchronizer System, one (1) stabilizing filter should suffice. The Stabilizing Filter is used in the first step of the protocol and becomes available as soon as its content is transferred to the Harvest Filter. Depending on the exclusion of cross-contamination, the Stabilizer Filter may need to be cleaned / rinsed before processing the next strain.
+ How are the filters cleaned?
Preferred method is to use 0.5M NaOH and / or (40khz) small ultrasonic cleaner. The ultra-sonic cleaner will ensure the filters are truly clean, while speeding up the process of cleaning. This will typically also take care of scaling. It is not recommended to descale the filters using an acid solution. Using an acid will at a minimum cause coloring/stains on the filter and repeated use of acids will ultimately damage the filters.
+ How are the filters sterilized?
Preferred method is to use 70% isopropyl alcohol, either use a spray or submerge the filters completely and afterwards let the filters dry in a laminar flow hood.
+ Why are the CES filters special?
It took 4 years to develop the CES system and the special micro-filters it relies on. Unlike traditional filters, the CES filters do not have 'round' or 'semi-round' pores/apertures however feature a special elongated design (slit) in order to prevent the filters from clogging while allowing L1’s to pass through with ease. In order to get the unique level of synchronization the CES provides, every single aperture in the filter needs to be ‘zero defect’.
CES-700: if you need between ~10 and less than 1k ~ 2k L1's with a short Harvest window or up to 5k~10k L1's with a larger Harvest window, the CES-700 will do the job. (this assumes ~1k gravid adults to be available to start with, (less is always possible)
CES-5000: if you need more than ~10k L1's with a short Harvest window or up to and excess of 100k or more per Harvest filter (multiple Harvest filters in parallel are always possible) then the CES-5000 is the preferred system.
Please note that the length of the 'Harvest Window' has a huge impact on the number of L1's harvested. The 'Gravid : L1' ratio is approximately 3-5 L1's per hour per available gravid nematode, after a 'lag' time of 7-8 hours (the time the egg's need to develop and hatch), assuming there are 'no' egg's to start with. Adding Serotonin at the Harvest step will give an initial 'burst' of 10-15 egg's per gravid nematode that can be harvested approximately 7-8 hours later. Adding Serotonin is a known method of stimulating the egg production and is used to get to the 'magic' 1 million+ number of perfect synchronized L1's. (See remarks CES protocol)